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EU-FP7: BASYNTHEC - Bacterial Synthetic Minimal Genomes for Biotechnology
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PROJEKT: The overarching goal of the project is to develop a model-based approach for engineering the Gram-positive bacterium Bacillus subtilis and to develop synthetic modules for the production of metabolites and proteins of interest.
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BaSynthec will combine computational and experimental biology approaches with novel high-throughput methodologies to reduce and modify a la carte the chromosome of Bacillus subtilis, a genetically tractable bacterium and one of the key microbes used as a Cell Factory in biotechnology.
Simpler B. subtilis strains with reduced energy consumption for self maintenance will be designed and constructed by removing some potentially expensive cellular processes. The cells with the lowest experimentally determined waste of energy and with industrially relevant phenotypes will be engineered to reroute the flux devoted to biomass formation through rational modifications of the complex metabolic regulations, and will be used as biotechnological platforms to plug in synthetic modules. For this purpose, BaSynthec will develop a model-driven approach to design and engineer the strains with predetermined features, with a particular focus on unrestricted metabolic activity and the plug-in of synthetic functional modules.
This strategy is based on the recent development of two complementary modelling approaches for B. subtilis:
- A genome-scale model of genetic and metabolic regulatory networks associated with a novel method called Resource Balance Analysis defining the formal background of model-based approaches for engineering strains
- The development of a new genome-scale metabolic model of B. subtilis which is the most complete and accurate that exists today
Two pathways of high biotechnological relevance will be used for establishing the proof-of-principle of the assembly of functional synthetic modules:
- The vitamin B5 biosynthetic pathway
- The secretion machinery for the export of extra-cellular enzymes
It is anticipated that validated simpler bacterial strains together with the modelling framework generated by BaSynthec will be used as generic biotechnological platforms to better control and exploit cell metabolism in industrial processes.
Homepage: http://basynthec.eu/
Project details:
Project Acronym: BASYNTHEC
Project Reference: 244093
Start Date: 2010-06-01
End Date: 2013-05-31
Duration: 36 months
Project Cost: 4.82 million euro
Project Funding: 2.99 million euro
Coordinator:
INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (FRANCE)
Participants:
ERNST-MORITZ-ARNDT-UNIVERSITÄT GREIFSWALD (GERMANY)
DSM NUTRITIONAL PRODUCTS LTD (SWITZERLAND)
ACADEMISCH ZIEKENHUIS GRONINGEN (NETHERLANDS)
THE UNIVERSITY OF CHICAGO (UNITED STATES)
INRA TRANSFERT S.A. (FRANCE)
NOVOZYMES A/S (DENMARK)
EIDGENÖSSISCHE TECHNISCHE HOCHSCHULE ZÜRICH (SWITZERLAND)
Contact:
Prof. Dr. Hecker
phone: ++49 3834 86 42 00
email: hecker [at] uni-greifswald.de Ernst-Moritz-Arndt-University Greifswald
Institute for Microbiology
Department of Microbial Physiology
F.-L.-Jahn-Str. 15
17489 Greifswald
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Naturwissenschaften 
Proteomics
für Projekte
uni-greifswald.de
